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Curriculum Vitae

Personal information
Name:
Academic position: Assistant Professor of Animal Biotechnology Laboratory of Genetics Department of Agricultural Biotechnology e-mail: [email protected]; Tel. +30 210 5294372 Researcher, Division of Immunology, Biomedical Sciences Research Center ‘Alexander Fleming’ 34 Fleming, Vari, 16672, Athens, Greece. e-mail: [email protected], Tel: +30 210 9656310 ext. 150 WEB: http://www.fleming.gr/en/investigators/Douni/index.html Education
1987-1991
Bachelor’s degree in Biology, Department of Biology (First Class Honours), University of Athens, Greece Ph.D. in Molecular Genetics. Thesis on “Studying the gene expression and biological role of Tumor Necrosis Factor (TNF) and its receptor p75 in transgenic mice”. Department of Biology, University of Athens, & Department of Molecular Genetics-Hellenic Pasteur Institute, Athens, Greece
Professional Experience
1998-2000
Postdoctoral fellow, Molecular Genetics Laboratory, Hellenic Pasteur Institute, Athens Greece Postdoctoral fellow, Institute of Immunology, B.S.R.C. ‘Al. Fleming’ Staff Research Scientist - Assistant Professor Level, Institute of Immunology, B.S.R.C. ‘Al. Fleming’ Staff Research Scientist – Associate Professor Level, Institute of Immunology, B.S.R.C. ‘Al. Fleming’ Researcher - Assistant Professor Level at the Institute of Immunology, B.S.R.C. ‘Al. Fleming’ Affiliated Researcher, Division of Immunology, B.S.R.C. ‘Al. Fleming’ Assistant Professor, Laboratory of Genetics, Department of Agricultural Biotechnology, AUA
Honors, awards, fellowships
1987-1991
Undergraduate fellowships, each year, Greek Governmental Institute of Scholarships National representative participating in FELASA (Federation of European Laboratory Animal Science Associations) working group for “refinement of methods for genotyping of genetically modified rodents”, 2008-2010 (www.felasa.eu/working groups)
Teaching Experience
Animal Biotechnology (theory and practicals to undergraduate students, 9th sem., AUA) (2005-present) Evolution (theory to undergraduate students, 8th semester, AUA) (2012-present) Invited lecturer at the post graduate Master course program organized by Medical School, University of Patras (2001-2009) Applied Physiology (post graduate Master course program, Medical School, University of Athens) (2010-present) Biotechnology and Applications in Agricultural Sciences (post graduate Master course program, Department of Agricultural Biotechnology, AUA) (2012-present) Diploma thesis supervision of 5 undergratuate students within my laboratory Master thesis supervisor of 2 students, within my laboratory Supervisor of 4 Ph.D. theses currently being performed in my laboratory
Technical Expertise in my laboratory
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Generation and characterization of transgenic and knockout mice ENU mutagenesis and screening of novel phenotypes Genome- wide genetic analysis using polymorphic markers (SNPs & SSLPs). Biochemistry, immunoprecipitation, production of recombinant proteins Histopathological analysis of mouse tissues including CNS Tissue culture practices: Stable and transient transfection of immortalized cell lines; isolation and culture of primary cells
Areas of Research Interests
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Generation and characterization of a novel genetic model of osteoporosis by overexpressing human RANKL in transgenic mice Development and biochemical characterization of novel RANKL inhibitors, evaluation in cellular assays and in our proprietary transgenic mouse model of RANKL-mediated osteoporosis Functional genetics for the identification of novel disease targets using random mouse mutagenesis, phenotyping, genetic analysis, identification and functional characterization of the causal mutation. Though this approach we have identified and are currently studying two novel genes: Ø A new mitochondrial member of the DnaJC family that causes a neuromuscular Ø A novel mitochondrial carrier member, SLC25, that causes ataxia and epilepsy in
Organization of conferences
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Member of the organizing and examination committee of the «International Course on Laboratory Animal Science» that takes place annually in B.S.R.C. “Al. Fleming” (2007-2011) Organizing member of the international «Workshop on Mouse Immune Phenotyping», 6-8 October 2009, Athens Member of the organizing committee of the 2nd Conference of Agricultural Biotechnology, October 2012, Athens
Other activities
E. Douni is a co-founder of Biomedcode Hellas, a Fleming spin-off for preclinical
evaluation studies and novel drug development and she has applied for one patent.

Reviewer at international scientific journals
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Publications in International Scientific Journals

1. Grell M, Douni E, Wajant H, Lohden M, Clauss M, Maxeiner B, Georgopoulos S,
Lesslauer W, Kollias G, Pfizenmaier K, and Scheurich P. (1995). The transmembrane
form of tumour necrosis factor (TNF) is the prime activating ligand of the 80 kDa TNF
receptor. Cell, 83, 793-802.

2. Douni E, Akassoglou K, Alexopoulou L, Georgopoulos S, Haralambous S, Hill S,
Kassiotis G, Kontoyiannis D, Pasparakis M, Plows D, Probert L, and Kollias G. (1995).
Transgenic and knockout analysis of the role of TNF in immune regulation and disease
pathogenesis. Journal of Inflammation, 47, 27-38.

3. Probert L, Akassoglou K, Alexopoulou L, Douni E, Haralambous S, Hill S, Kassiotis G,
Kontoyiannis D, Pasparakis M, Plows D, and Kollias G. (1996). Dissection of the
pathologies induced by transmembrane and wild-type tumour necrosis factor in
transgenic mice. Journal of Leukocyte Biology, 59, 518-525.

4. Pasparakis M, Alexopoulou L, Douni E, and Kollias G. (1996). Tumour necrosis factors
in immune regulation: everything that’s interesting is … new! Cytokine and Growth
Factor Reviews, 7, 223-229.

5. Kusters S, Tiegs G, Alexopoulou L, Pasparakis M, Douni E, Kunstle G, Bluethmann H,
Wendel A, Pfizenmaier K, Kollias G, and Grell M. (1997). In vivo evidence for a
functional role of both tumor necrosis factor (TNF) receptors and transmembrane TNF in
experimental hepatitis. European Journal of Immunology, 27, 2870-2875.

6. Douni E, and Kollias G. (1998). A critical role of the p75TNF-R in organ inflammation
independent of TNF, LTα or the p55 TNF-R. Journal of Experimental Medicine, 188,
1343-1352.

7. Kollias G, Douni E, Kassiotis G, and Kontoyiannis D. (1999). On the role of tumor
necrosis factor and receptors in models of multiorgan failure, rheumatoid arthritis,
multiple sclerosis and inflammatory bowel disease. Immunological Reviews, 169, 175-
194.

8. Kollias G, Douni E, Kassiotis G, and Kontoyiannis D. (1999). The function of tumour
necrosis factor and receptors in models of multi-organ inflammation, rheumatoid arthritis,
multiple sclerosis and inflammatory bowel disease. Ann. Rheum. Dis., 58, 132-139.

9. Holtmann M, Douni E, Schutz M, Mudter J, Lehr H, Gerspach J, Scheurich P, Galle P,
Kollias G. and Neurath M. (2002). TNF-R2 is upregulated on lamina propria
mononuclear cells in Crohn`s disease and promotes experimental colitis in vivo.
European Journal of Immunology, 32:3142-3151.

10. Akassoglou Κ, Douni Ε, Bauer J, Lassmann H, Kollias G, and Probert L. (2003). The
p75TNF Receptor Independently Signals Pro-inflammatory Effects of TNF at the CNS
Vasculature and the Development of Spontaneous Inflammatory Ischaemia in
Transgenic Mice. PNAS, 100:709-714.

11. Douni E, Sfikakis P, Haralambous S, Fernandez P, and Kollias G. (2004). Attenuation of
inflammatory polyarthritis in TNF transgenic mice by diacerein: comparative analysis
with dexamethasone, methotrexate and anti-TNF protocols. Arthritis Research &
Therapy, 6(1):R65-R72.

12. Thwin MM, Douni E, Aidinis V, Kollias G, Kodama K, Sato K, Satish RL, Mahendran R,
and Gopalakrishnakone P. (2004). Effect of Phospholipase A2 Inhibitory Peptide on Inflammatory Arthritis in a TNF Transgenic Mouse Model: A Time Course Ultrastructural
Study. Arthritis Research & Therapy, 6(3):R282-94.

13. Alexopoulou L, Kranidioti K, Xanthoulea S, Denis M, Kotanidou A, Douni E, Blackshear
PJ, Kontoyiannis DL, and Kollias G. (2006). Transmembrane TNF protects mutant mice
against intracellular bacterial infections, chronic inflammation and autoimmunity.
European Journal of Immunology, 36(10):2768-80.
14. Aidinis V, Chandras C, Manoloukos M, Thanassopoulou A, Kranidioti K, Armaka M, Douni E, Kontoyiannis DL, Zouberakis M, and Kollias G; Mugen NoE consortium.
(2008). MUGEN mouse database; animal models of human immunological diseases.
Nucleic Acids Res;36 D1048-54.

15. Thwin MM, Douni E, Arjunan P, Kollias G, Kumar PV, Gopalakrishnakone P. (2009).
Suppressive effect of secretory phospholipase A2 inhibitory peptide on IL-1-β-induced
matrix metalloproteinases production in rheumatoid synovial fibroblasts, and its
antiarthritic activity in hTNFtg mice. Arthritis Research & Therapy; 11:R138.
16. Morgan H, Beck T, Blake A, Gates H, Adams N, Debouzy G, Leblanc S, Lengger C, Maier H, Melvin D, Meziane H, Richardson D, Wells S, White J, Wood J; EUMODIC
Consortium, de Angelis MH, Brown SD, Hancock JM, Mallon AM. (2010).
EuroPhenome: a repository for high-throughput mouse phenotyping data. Nucleic
Acids Res; 38:D577-85.

17. Douni E*, Rinotas V, Makrinou E, Zwerina J, Penninger JM, Eliopoulos E, Schett G,
Kollias G. (2012). A RANKL G278R mutation causing osteopetrosis identifies a
functional amino acid essential for trimer assembly in RANKL and TNF. Hum Mol
Genet.; 21(4):784-98. (*Corresponding author)

Book chapters

1. Kollias G, Kontoyiannis D, Douni E, Kassiotis G. (2002). The role of TNF/TNFR in
organ-specific and systemic autoimmunity: implications for the design of optimized `anti-
TNF` therapies. In Curr. Dir. Autoimmun.: Signal transduction pathways in
autoimmunity, Eds. A. Theofilopoulos, Karger Press; 5:30-50.

2. Douni E, Alexiou M, Kollias G. (2004). Genetic engineering in the mouse: Tuning
TNF/TNFR expression. In Methods in Molecular Medicine: Tumor Necrosis Factor.
Eds A. Corti and P. Ghezzi. Humana Press Inc; 98: p139-171.

3. Douni E, Armaka M, Kontoyiannis DL, Kollias G. (2007). Functional genetic and
genomic analysis of modeled arthritis. In Adv Exp Med Biol.: Osteoimmunology.
Interactions of the Immune and Skeletal Systems. Ed Y Choi, Springer; 602:33-42.

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