Guru/Journal of Engineering, Science and Management Education/Vol. 2, 2010/73-77MICROBIAL STUDIES ON SOME METAL COMPLEXES WITH TETRACYCLINE Pranay Guru* Received July 22, 2010, Revised July 23, 2010 ; Accepted Aug. 26, 2010Abstract RESULTS AND DISCUSSIONS The present paper deals with the microbial studies of Ni(II)
In general all the tested complexes showed higher toxicity
and Co(II) complexes with antibiotic drug tetracycline. The
against bacterial and fungi under study . composition Ni (C H N O ) WO 3H O and Co (C H N Antibacterial Activity O )WO 4H O complexes has been suggested on the basis of
From the Tables 1 and 2 it is concluded that complex S1 has
elemental analysis and molar conductance values. The
exhibits maximum zone of inhibition against Shigella
microbial studies of these complexes were studied on
flexneri at the concentration of 0.1 M. Even at the
pathogenic bacteria using gram +ve (Bacillus subtilis and
concentration of 0.01 M it has shown good zone of inhibition
Staphylococcus aureus) and gram -ve (Shigella flexneri,
in compression to other tested complexes. Against
Salmonella typhosa, Escherichia coli) and some fungi
Salmonella typhosa good antibacterial activity was observed
(Aspergillus flavus, Fusarium oxysporum, Chrysosporium
against almost all the tested complexes. pannicale, Alternaria solani, Candida albicans ).
Complex S2 shows maximum zone of inhibition Salmonella
Keywords-Tetracycline, Complexes, Microbial Activity,
typhosa. Similarly, against Escherichia- coli maximum
inhibitory effect were produced by complex S2. Bacillus
subtitles was found to be more susceptible against complex
The use of raw extract of plants for the amelioration of human
S2. Complex S2 found to be more active and shown higher
sufferings without knowing their chemical constituent and
zone of inhibition against Staphylococcus aureus in
active principles is well-known. Such importance of plant
comparison to S1. On comparing the anti-bacterial efficacy of
products and their utility encouraged human being to find out
these complexes, it is concluded that complex S2 gave
the mysteries and marvels of biological processes for
attaining this aim. It was noticed that several metal complexes
For the sake of comparison of the antibacterial properties of
with drugs posses more medicinal importance as compared to
these complexes the zone of inhibition have been graphically
In the present age a large number of common diseases of man
Anti Fungal Activity
and animals which are caused by bacterial infections are
Study of anti-fungal activity of complexes S1, S2 was carried
treated by antimicrobial drug and may require a long
out against selected five fungi namely Aspergillus flavus,
treatment for their cure. However, the present study shows
Candida albicans, Alternaria solani, Fusarium oxysporum
that some metal-complexes could be of better use against
and Chrysosporium pannicale at varying concentration of
these diseases as these complexes can easily be metabolized
complexes 0.1M, 0.5 M and 0.01 M respectively and the
into man or animal systems along-with their quick curative
result are recorded in terms of zone of inhibition which also
.In doing so we have carried out microbial studies of
includes the diameter of filter paper disc (6mm).
Ni (II) and Co(II) with tetracycline.
From Table 3 it is observed that at the concentration of 0.1M
the complex S2 shows maximum zone of inhibition against
Microbial studies of the synthesized complexes were
Aspergillus flavus .Similarly good inhibitory efficacy was
performed at Department of Microbiology, Dr H.S. Gour
also observed at the same concentration of complexes S2
University Sagar (M.P.) and Govt. Veterinary college
against Aspergillus flavus. Against Candida albicans at the
Jabalpur (M.P.) using paper disc method against the
concentration of 0.1M complex S2 have shown maximum
following pathogenic bacteria using gram +ve (Bacillus
activity but similarly, considerable zone of inhibition were
subtilis and Staphylococcus aureus) and gram -ve (Shigella
also recoded in case of complexes . It is evident from Table 3
flexneri, Salmonella typhosa, Escherichia coli) and some
that even at the concentration of 0.01 M both the complexes
fungi (Aspergillus flavus, Fusarium oxysporum,
were found to be active against Candida albicans. Maximum
Chrysosporium pannicale, Alternaria solani, Candida
zone of inhibition were recorded these complexes at the
albicans). The synthesized complexes were screened for the
concentration of 0.1M against Alternaria solani. The
antibacterial and antifungal activity using standard paper disc
complexes at the concentration of 0.5 M have also gave
promising results. The complex S2 at the concentration of 0.1
* Department of Engineering Chemistry, People's College of Research & Technology, Bhopal (M. P.) India, [email protected]Journal of Engineering, Science and Management Education
M produced maximum zone of inhibition against Fusarium
Table 3 : Antifungal Activity of Synthesized Complexes
oxysporum. Microorganism Chrysosporium pannicale was
Stain of Fungi/
found susceptible against all the complexes tested at their
Zone of Inhibition
concentration of 0.1M and 0.5 Complex S2 was found to
posses good antifungal activity at 0.1 M concentration.
The results are presented in figures 6-10.Antimicrobial
properties of the original drug against selected
microorganism were also compared. It could be observed that
synthesized complex have shown promising result compared
to commercial original drug tetracycline.
From the present study it is concluded that the complexes
Alternaria solani -
used have shown considerable antimicrobial activity and are
found more effective against tested bacteria comparative to
fungi. Results also indicate that inhibitory power of the
complexes decreases with the increase of their concentration.
oxysporum Table-1 : Complexes of Different Metals Were Chrysosporium Marked Has S1 ,S2 as follows pannicale
Including diameter of filter-paper disc (6mm)
H N O ) WO 4H O, S2 =Co (C H N O ) WO 3H O
Table-2 : Antibacterial Activity of Synthesized Complexes Stain of Bacteria/ Zone of Inhibition Concentration Inhibition Bacteria Shigella flexneri Concentration Salmonella
S1 = Ni (C H N O ) WO 4H O, S2 =Co (C H N O ) WO 3H O
typhosa Graph 1 : Comparative Antibacterial Activity Of Complexes Against Shigella Flexneri Escherichia- coli Bacillus subtilis Zone of 15 Inhibition Staphylococcus aureus Concentration
Including diameter of filter-paper disc (6mm)
S1 = Ni (C H N O ) WO 4H O, S2 =Co (C H N O ) WO 3H O
S1 = Ni (C22 H24 N2 O8) WO4 4H2O, S2 =Co (C22 H24 Graph 2 : Comparative antibacterial activity of complexes against Salmonella typhosa Journal of Engineering, Science and Management EducationZone of 10 Inhibition Inhibition 8 Concentration Concentration Graph 3 : Comparative antibacterial activity of Graph 6 : Comparative antifungal activity of complexes against Escehrichia-coli complexes against Aspergillus flavus Inhibition Inhibition Concentration Concentration Graph 4 : Comparative antibacterial activity of Graph 7 : Comparative antifungal activity of complexes against Bacillus subtilis complexes against Candida albicans Inhibition Inhibition Concentration Concentration Graph 5 : Comparative antibacterial activity of Graph 8 : Comparative antifungal activity of complexes against Staphylococcus aureu complexes against Alternaria solani Journal of Engineering, Science and Management Education
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Nephrol Dial Transplant (1997) 13: 2402–2406 Continuing Nephrological Education (CNE) Iatrogenic hyperkalaemia—points to consider in diagnosis and management Kostas C. Siamopoulos, Moses Elisaf and Kostas KatopodisDepartment of Internal Medicine, Division of Nephrology, University Hospital of Ioannina, Greece Introduction 6.1 mmol/l ) and renal impairment (serum creatinine160 mmol/l,
PORTARIA No- 14, DE 15 DE JANEIRO DE 2010 O Secretário de Atenção à Saúde, no uso de suas atribuições, Considerando a necessidade de se estabelecer parâmetros sobre o Hipoparatireoidismo no Brasil e de diretrizes nacionais para diagnóstico, tratamento e acompanhamento dos indivíduos com esta doença; Considerando que os Protocolos Clínicos e Diretrizes Terapêuticas (PCDT) são re